2019-nCoV a me B.1.617.2 Kokoke Ho'āʻo Nucleic Acid (PCR-fluorescence probe method)
Nā pono
Laʻana: Nasopharyngeal swabs kanaka, oropharyngeal swabs, sputum, alveolar lavage fluid specimens.
Hōʻike: 12×8T/Kit;4×24T/Kit;
Nā kūlana mālama a me ka manawa kūpono: ʻAʻole wehe ʻia ka pahu: E kūʻai ma waena o -25 ℃ a 8 ℃ mai ka mālamalama, kūpono no 18 mahina.
Peila: FAM (no N gene), VIC (no T478K), ROX (L452R), CY5 (no ka maʻamau o loko)
Nā hiʻohiʻona
N a me T478K, L452R pahuhopu:ʻO nā pahuhopu ʻekolu e hoʻomaikaʻi i ka helu ʻike maikaʻi, e hoʻemi nui i ka helu o ka ʻike ʻole.
ʻO ka mana maikaʻi he ʻāpana pseudovirus:ʻO ka hoʻohālikelike ʻana i ka hoʻolālā coronavirus hou a ke pāʻani nei i kahi kuleana mana maoli.
Nā palena ʻike liʻiliʻi:400 kope/mL no 2019-NCOV, 400 kope/mL no 2019-NCOV T478K mutation a me 1000 kope/mL no 2019-NCOV L452R mutation.
Ua hoʻonohonoho ʻia nā mana ʻino a maikaʻi, a ua hoʻokomo ʻia kahi ʻōnaehana maʻamau i loko, hiki ke hōʻemi nui i nā hopena hoʻopunipuni i hana ʻia e ka hana
Nā pōmaikaʻi o ka pauka maloʻo maloʻo:maʻalahi i ka lawe ʻana, paʻa ka hana reagent, ʻaʻohe pilikia hoʻoheheʻe-hau.
Hoʻonohonoho mea kani
Ua hoʻokomo ʻia nā paipu pane/pāpā i loko o ka mea kani a ua koho ʻia nā kaha ʻehā no FAM, VIC, ROX a me CY5 no kēlā me kēia laʻana.ʻO nā ʻōnaehana polokalamu kikoʻī penei:
Hōʻike huahana
8X12
24X2
HS33- 12X8T/Puke
HS33- 4X24T/Puke
